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李彬

江苏省农科院兽医所-李彬.jpg

    

李彬,博士,研究员,博士生导师,江苏省农业科学院科研管理处处长、兽医研究所所长。国家青年人才计划、江苏省杰出青年基金获得者,农业农村部兽用生物制品工程技术重点实验室主任。南京农业大学、河北农业大学、西藏农牧学院、江苏大学等高校研究生导师。江苏省十大青年科技之星,江苏省产业教授,江苏省“333高层次人才培养工程”中青年科学技术带头人,江苏省“六大人才高峰”资助对象,江苏省科技社团(学会)优秀青年人才,美国斯坦福大学访问学者,教育部学位论文评议专家。兼任江苏省畜牧兽医学会理事长,中国畜牧兽医学会兽医公共卫生学分会常务理事,动物传染病学分会理事,中国微生物学会兽医微生物学专委会委员,江苏省兽医免疫学常务委员,江苏省农业科学院学术委员会副主任、青工委主任、欧美同学会副会长。

主要研究领域和方向:动物腹泻病防控技术研究,重要猪病感染与免疫的分子机制、新型疫苗和诊断试剂开发。近年来,主持国家重点研发计划子课题3项,国家自然科学基金5项、江苏省自然科学基金2项,江苏省农业科技自主创新资金项目3项。以第一或通讯作者在Nat Commun、PNAS、mBio、EID、JVI、JBC、JCM、Virology等杂志发表论文70余篇;获国家发明专利授权6项。获中国产学研合作创新奖,中国技术市场协会第十届“金桥奖”二等奖,中国发明协会成果创新奖二等奖、江苏省青年科技奖,江苏农业科技奖卓越人才奖、江苏省农业丰收奖二等奖等奖项。J Virol、J Biol Chem、Vet Microbiol等杂志审稿人。

联系电话:025-84390748   电子邮箱:libinana@126.com

 

一、科研项目

1.   国家自然科学基金面上项目,PEDV调控基质金属酶13促进病毒吸附的分子机制研究,项目编号:32272996,资助年限:2023.01-2026.12,资助金额:55万元;

2.   十四五国家重点研发计划子课题,畜禽重要冠状病毒逃逸或拮抗宿主抗病毒天然免疫的分子机制。项目编号:2021YFD1801104,资助年限:2021.12-2024.11,资助金额:225万元;

3.   国家自然科学基金重点专项,非洲猪瘟病毒感染诱导凝血的分子机制研究,项目编号:31941013,资助年限:2020.01-2022.12,资助金额:100万元;

4.   江苏省自然科学基金杰出青年基金,与PEDV UTR互作的RNA结合蛋白的筛选及其在病毒复制与致病中的作用研究。项目编号:BK20190003,资助年限:2019.7-2022.6,资助金额:100万元;

5.   江苏省农业科技自主创新资金,畜禽重要疫病多联多价疫苗创制。项目编号:CX(19)2020,资助年限:2019.7-2021.6,资助金额:140万元;

6.   国家自然科学基金面上项目,细胞骨架蛋白ADD1对A群轮状病毒的抑制作用及其分子机制,项目编号:31872481,资助年限:2019.01-2022.12,资助金额:59万元;

7.   国家重点研发计划,猪呼吸道病原微生物共感染与协同致病机理。项目编号:2018YFD0500101,资助年限:2018.06-2020.12,资助金额:40.5万元;

8.   第十三批六大人才高峰高层次人才资助项目,猪流行性腹泻疫苗与检测试剂盒创制。项目编号:NY-45,资助年限:2016.07-2019.06,资助金额:4万元;

9.   江苏省地方标准,猪萨佩罗病毒(Porcine Sapelovirus, PSV)诊断技术。项目编号:5511611,资助年限:2016-2018,资助金额:5万元;

10. 江苏省地方标准,猪delta冠状病毒诊断技术。项目编号:5511611,资助年限:2016-2018,资助金额:2.5万元;

11. 国家重点研发计划,猪流行性腹泻病毒的遗传演化与免疫逃逸的分子基础。项目编号:2016YFD0500101,资助年限:2016.07-2020.12,资助金额:110万元;

12. 国家自然科学基金面上项目,猪肺炎支原体与PRRSV混合感染激活IL-1β的分子机制及生物学意义。项目编号:31472204,资助年限:2015.1-2018.12,资助金额:82万元;

13. 江苏省双创计划,规模化猪场绿色健康新型养殖模式的创建。2015.7-2017.6,资助金额:15万元;

14. 江苏省农业科学院基本科研业务专项,猪肺炎支原体和PRRSV共感染激活IL-1β的分子机制。2015.01-2015.12,资助金额:20万元;

15. 江苏省自然科学基金面上项目,猪肺炎支原体与PRRSV混合感染激活IL-1β的分子机制研究。项目编号:BK20131330,资助年限:2013.7-2016.6,资助金额:10万元;

16. 江苏省农业科技自主创新资金,猪流行性腹泻病毒系列诊断试剂盒研制。项目编号:CX(13)3069,资助年限:2013.7-2015.6,资助金额:40万元;

17. 国家自然科学基金青年基金,猪肺炎支原体和猪繁殖与呼吸综合征病毒混合感染的分子机制研究,项目编号:31001066,资助年限:2011.1-2013.12,资助金额:19万元;

18. 江苏省农业科技自主创新资金,猪博卡病毒新型分子诊断及抗体检测技术研究,项目编号:CX(11)4043,资助年限:2011.7-2013.6,资助金额:15万元。 

 

二、科研成果

获中国产学研合作创新奖1项

中国技术市场协会第十届“金桥奖”二等奖1项

中国发明协会成果创新奖二等奖

江苏省青年科技奖

江苏农业科技奖卓越人才奖

江苏省农业丰收奖二等奖

南京市自然科学优秀学术论文奖二等奖1项,三等奖1项,优秀奖1项,

江苏省农科院第一届高亮之科技论文奖一等奖。

 

三、获得专利授权

1.   2022年,重组蛋白和猪流行性腹泻疫苗组合物,专利号:ZL202110546536.3,第1完成人。

2.   2020年,猪流行性腹泻病毒双抗体夹心ELISA定量检测试剂盒及其应用,专利号:ZL201710269905.2,第1完成人。

3.   2020年,PEDV JS2008株全长感染性cDNA的构建方法及应用,专利号:ZL201710674137.9,第2完成人。

4.   2020年,用于检测猪Delta冠状病毒抗体的多肽、制备方法及其应用,专利号:ZL201611007940.9,第1完成人。

5.   2017年,猪流行性腹泻病毒强毒株和弱毒株鉴别诊断用引物及其检测试剂盒,专利号:ZL. 201410696032.X,第4完成人。

6.   2014年,一种猪瘟病毒重组E2 蛋白及其IgM 抗体ELISA 检测试剂盒,专利号:ZL 201210013152.6,第4完成人。

7.   2013年,一种检测猪Hokovirus的PCR方法,专利号:ZL201110063355.1,第1完成人。

8.   2013年,一种用于检测猪Hokovirus核苷酸片段的引物和探针序列,专利号:ZL201110063354.7,第1完成人。

9.   2012年,一种用于检测猪博卡病毒核苷酸片段的引物和探针序列,专利号:ZL201110063287.9,第1完成人。

10. 2011年,人工合成的修饰型高致病性猪繁殖与呼吸综合征病毒ORF5基因及应用,专利号:ZL200810048768.0,第2完成人。

 

四、发表主要论文

1.   Baochao Fan, Qi Peng, Shiying Song, Danyi Shi, Xue Zhang, Weilu Guo, Yunchuan Li, Jinzhu Zhou, Xuejiao Zhu, Yongxiang Zhao, Rongli Guo, Kongwang He, Huiying Fan, Siyuan Ding, Bin Li. Nonstructural Protein 1 of Variant PEDV Plays a Key Role in Escaping Replication Restriction by Complement C3. J Virol. 2022 Aug 29; e0102422. doi: 10.1128/jvi.01024-22.

2.   Baotai Zhang, Shuqing Zhao, Chunyan Zhong, Li Xiao, Ai Yan, Tao Xue, Jin Huang, Jinzhu Zhou, Qi Peng, Rongli Guo, Baochao Fan, Chuanmin Liu, Yanxiu Ni, Xing Zhu, Jianhong Shu, Yinhe Zha, Jin Chen, Jizong Li, Bin Li. Comparison of pathogenicity of porcine deltacoronavirus CZ2020 from cell culture and intestinal contents in 27-day-old piglets. Microb Pathog. 2022 Sep; 170: 105723. doi: 10.1016/j.micpath.2022.105723. 

3.   Qi Peng, Xue Zhang, Jizong Li, Wenlong He, Baochao Fan, Yanxiu Ni, Maojun Liu, Bin Li. Comprehensive analysis of codon usage patterns of porcine deltacoronavirus and its host adaptability. Transbound Emerg Dis. 2022 May 8. doi: 10.1111/tbed.14588.

4.   Weiwei Ji, Qi Peng, Xueqiong Fang, Zehou Li, Yaxin Li, Cunfa Xu, Shuqing Zhao, Jizong Li, Rong Chen, Guoxiang Mo, Zhanyong Wei, Ying Xu, Bin Li, Shuijun Zhang. Structures of a deltacoronavirus spike protein bound to porcine and human receptors. Nature Communications, 2022 Mar 18; 13(1):1467.

5.   Qi Peng, Xue Zhang, Baochao Fan, Yunchuan Li, Shuqing Zhao, Weilu Guo, Wenlong He, Yongxiang Zhao, Yanxiu Ni, Maojun Liu, Rongmei Fei, Bin Li. Evaluation of the transcriptional regulatory efficacy of transcription regulatory sequences of porcine epidemic diarrhea virus. Vet Microbiol, 2022 Feb 17; 267: 109376.

6.   Juhee Son, Shimeng Huang, Qiru Zeng, Traci L. Bricker, James Brett Case, Jinzhu Zhou, Ruochen Zang, Zhuoming Liu, Xinjian Chang, Tamarand L. Darling, Jian Xu, Houda H. Harastani, Lu Chen, Maria Florencia Gomez Castro, Yongxiang Zhao, Hinissan P. Kohio, Gaopeng Hou, Baochao Fan, Beibei Niu, Rongli Guo, Paul W. Rothlauf, Adam L. Bailey, Xin Wang, Pei-Yong Shi, Elisabeth D. Martinez, Steven L. Brody, Sean P. J. Whelan, Michael S. Diamond, Adrianus C. M. Boon, Bin Li, Siyuan Ding. JIB-04 Has Broad-Spectrum Antiviral Activity and Inhibits SARS-CoV-2 Replication and Coronavirus Pathogenesis. mBio, 2022 Jan 18;13(1):e0337721

7.   Jun Gu , Shuaifeng Zhang , Xuefeng Xia , Xuehan Zhang , Baochao Fan , Junming Zhou , Haodan Zhu , Wei Wang , Xianghui Qi , Li Li , Bin Li . An edible kanamycin sulfate cross-linked cellulose active against multiple pathogenic bacteria. Int J Biol Macromol. 2022 Jan 1;194:435-444

8.   Danyi Shi, Baochao Fan, Bing Sun, Jinzhu Zhou,Yongxiang Zhao, Rongli Guo, Zengjun Ma, Tao Song, Huiying Fan, Jizong Li, Li Li, BinLi. LDH nanoparticle adjuvant subunit vaccine induces an effective immune response for porcine epidemic diarrhea virus. Virology. 2022, 58-64

9.   Haodan Zhu , Xinjian Chang , Jinzhu Zhou , Dandan Wang , Junming Zhou , Baochao Fan , Yanxiu Ni , Jie Yin , Lixin Lv , Yongxiang Zhao , Kongwang He Bin Li. Co-infection analysis of bacterial and viral respiratory pathogens from clinically healthy swine in Eastern China. Vet Med Sci.2021. 7(5):1815-1819

10. Wei Wang, Jizong Li, Baochao Fan, Xuehan Zhang, Rongli Guo, Yongxiang Zhao, Junming Zhou, Jinzhu Zhou, Dongbo Sun and Bin Li. Development of a Novel Double Antibody Sandwich ELISA for Quantitative Detection of Porcine Deltacoronavirus Antigen. Viruses 2021, 13(12), 2403

11. Qi Wu, Jizong Li, Wei Wang, Jinzhu Zhou, Dandan Wang, Baochao Fan, Xuehan Zhang, Dongbo Sun, Ga Gong, Sizhu Suolang, Bin Li. Next-Generation Sequencing Reveals Four Novel Viruses Associated with Calf Diarrhea. Viruses 2021, 13(10), 1907

12. Jinzhu Zhou, Shimeng Huang, Baochao Fan, Beibei Niu, Rongli Guo, JunGu, Song Gao, BinLi. iTRAQ-based proteomanalysis of porcine group A rotavirus-infected porcine IPEC-J2 intestinal epithelial cells. J Proteomics, 2021 Sep 30;248:104354.

13. Haodan Zhu, Xinjian Chang,Jinzhu Zhou , Dandan Wang, Junming Zhou, Baochao Fan, Yanxiu Ni, Jie Yin , Lixin Lv , Yongxiang Zhao , Kongwang He, Bin Li. Co-infection analysis of bacterial and viral respiratory pathogens from clinically healthy swine in Eastern China. Vet Med Sci.2021 Sep; 7(5):1815-1819. doi: 10.1002/vms3.533.

14. Jizong Li, Jinzhu Zhou, Shuqing Zhao, Rongli Guo, Chunyan Zhong, Tao Xue, Qi Peng, Baotai Zhang, Baochao Fan, Chuanmin Liu, Yanxiu Ni, Lili Ren, Xing Zhu, Bin Li. Pathogenicity, infective dose and altered gut microbiota in piglets infected with porcine deltacoronavirus. Virology. 2021 14;567:26-33

15. Xuejiao Zhu, Baochao Fan, Junming Zhou, Dandan Wang, Huiying Fan and Bin Li*.A High-Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus In Vitro. Front. Vet. Frontiers in Veterinary Science. 2021;8:719859

16. Jizong Li, Leilei Yang, Li Mao, Wen liang Li, Min Sun, Chuanmin Liu, Tao Xue, Wenwen Zhang, Maojun Liu, Bin Li. Caprine parainfluenza virus type 3 N protein promotes viral replication via inducing apoptosis. Vet microbiology. 259 (2021) 109129.

17. Baochao Fan, Jie Sun, Lin Zhu, Jinzhu Zhou, Yongxiang Zhao, Zhengyu Yu, Bing Sun, Rongli Guo, Kongwang He, Bin Li. Development of a Novel Double Antibody Sandwich Quantitative Enzyme-Linked Immunosorbent Assay for Detection of Porcine Epidemic Diarrhea Virus Antigen. Front Vet Sci. 2020, 7: 540248.

18. Guangzhi Zhang*, Bin Li*, Dongwan Yoo*, Tong Qin, Xiaodong Zhang, Yaxiong Jia, Shangjin Cui. Animal coronaviruses and SARS-CoV-2. Transbound Emerg Dis. 2020; doi: 10.1111/tbed.13791.

19. Xu X, Du L, Fan B, Sun B, Zhou J, Guo R, Yu Z, Shi D, He K, Li B. A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets. Arch Virol. 2020, 165(6):1299-1309.

20. Rongli Guo, Baochao Fan, Xinjian Chang, Jinzhu Zhou, Yongxiang Zhao, Danyi Shi, Zhengyu Yu, Kongwang He*, Bin Li*. Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China. Virology, 2020, 545:24-32.

21. Fan B, Zhu L, Chang X, Zhou J, Guo R, Zhao Y, Shi D, Niu B, Gu J, Yu Z, Song T, Luo C, Ma Z, Bai J, Zhou B, Ding S, He K*, Li B*. Mortalin restricts porcine epidemic diarrhea virus entry by downregulating clathrin-mediated endocytosis. Vet Microbiol. 2019, 239:108455.

22. Fan B, Jiao D, Zhang R, Zhou J, Guo R, Yu Z, Shi D, Zhao Y, Gu J, Niu B, Ma Z, Gao S, He K, Li B*. Origin and epidemic status of porcine epidemic diarrhea virus variants in China. Transbound Emerg Dis. 2020, 67(3):1364-1370.

23. Luo L, Wang S, Zhu L, Fan B, Liu T, Wang L, Zhao P, Dang Y, Sun P, Chen J, Zhang Y, Chang X, Yu Z, Wang H, Guo R, Li B*, Zhang K*. Aminopeptidase N-null neonatal piglets are protected from transmissible gastroenteritis virus but not porcine epidemic diarrhea virus. Scientific Reports, 2019, 9(1):13186.

24. Ren L*, Ding S*#, Song Y*, Li B*, Co J, Ramanathan M, Amieva MR, Khavari PA, and Greenberg HB#. Profiling of rotavirus 3’ UTR binding proteins reveals ATP synthase subunit ATP5B as a novel host factor that supports late stage virus replication. J Biol. Chem. 2019, 294(15): 5993-6006.

25. Du L, Yu Z, Pang F, Xu X, Mao A, Yuan W, He K*, Li B*. Targeted Delivery of GP5 Antigen of PRRSV to M Cells Enhances the Antigen-Specific Systemic and Mucosal Immune Responses. Front Cell Infect Microbiol. 2018, 25; 8:7. doi: 10.3389/fcimb. 

26. Bin Li, Luping Du, Zhengyu Yu, Bing Sun, Xiangwei Xu, Baochao Fan, Rongli Guo, Wanzhe Yuan, Kongwang He. Poly (D, L-lactide-co-glycolide) nanoparticle-entrapped vaccine induces a protective immune response against porcine epidemic diarrhea virus infection in piglets. Vaccine, 2017.15;35(50):7010-7017.

27. Bin Li, Siyuan Ding, Ningguo Feng, Nancie Mooney, Yaw Shin Ooi, Lili Ren, Jonathan Diep,   Marcus R. Kelly, Linda L. Yasukawa, John T. Patton, Hiroyuki Yamazaki, Tomoaki Shirao, Peter K. Jackson, and Harry B. Greenberg*. Drebrin restricts rotavirus entry by inhibiting dynamin-mediated endocytosis. Proc Natl Acad Sci USA. 2017. 114(18):E3642-E3651.

28. Lin H, Li B, Chen L, Ma Z, He K, Fan H. Differential Protein Analysis of IPEC-J2 Cells Infected with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strains Elucidates the Pathogenesis of Infection. J Proteome Res. 2017, 16(6):2113-2120.

29. Fan B, Jiao D, Zhao X, Pang F, Xiao Q, Yu Z, Mao A, Guo R, Yuan W, Zhao P, He K, Li B*. Characterization of Chinese Porcine Epidemic Diarrhea Virus with Novel Insertions and Deletions in Genome. Sci Rep. 2017, 7:44209.

30. Du L, Pang F, Yu Z, Xu X, Fan B, Huang K, He K, Li B*. Assessment of the efficacy of two novel DNA vaccine formulations against highly pathogenic Porcine Reproductive and Respiratory Syndrome Virus. Sci Rep. 2017, 7: 41886;

31. Fan B, Yu Z, Pang F, Xu X, Zhang B, Guo R, He K, Li B*. Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China. Virology. 2017 500, 50-61.

32. Ding S, Mooney N, Li B, Kelly MR, Feng N, Loktev AV, Sen A, Patton JT, Jackson PK, Greenberg HB. Comparative Proteomics Reveals Strain-Specific β-TrCP Degradation via Rotavirus NSP1 Hijacking a Host Cullin-3-Rbx1 Complex. PLoS Pathog. 2016, 5;12(10):e1005929.

33. Du L1, Bin Li (co-first author), Xu X, Sun B, Pang F, Wen L, Huang K, He K. Adsorption of a porcine reproductive and respiratory syndrome virus DNA vaccine candidate onto biodegradable nanoparticles improves immunogenicity in mice. Arch Virol. 2015, 160(6):1543-7.

34. Bin Li, Luping Du, Xiangwei Xu, Bing Sun, Zhengyu Yu, Zhixin Feng, Maojun Liu, Yanna Wei, Haiyan Wang, Guoqing Shao, Kongwang He.Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Virus research, 2015, 196,60-69.

35. Bin Li, Luping Du, Bing Sun, Zhengyu Yu, Maojun Liu, Zhixin Feng, Yanna Wei, Haiyan Wang, Guoqing Shao, Kongwang He. Transcription Analysis of the Porcine Alveolar MacrophageResponse to Mycoplasma hyopneumoniae. PLoS ONE 2014, 9(8): e101968.

36. Bin Li*, Du L, Sun B, Yu Z, Wen L, Zhang X, Guo R, Ni Y, Hu Y, Zhou J, Zhu H, Lv L, Yu Y, Wang X, He K*. Monoclonal antibodies against Nsp2 protein of the highly pathogenic porcine reproductive and respiratory syndrome virus. Monoclon Antib Immunodiagn Immunother. 2013, 32(5): 362-365.

37. Bin Li*, Bing Sun, Lu-ping Du, Ai-hua Mao, Li-bin Wen, Yan-xiu Ni, Xue-han Zhang, Kong-wang He*. Development of a loop-mediated isothermal amplification assay for the detection of porcine hokovirus. J Virol Methods. 2013, 193(2): 415-418.

38. Bin Li*, Haofei Liu, Kongwang He, Rongli Guo, Yanxiu Ni, Luping Du, Libin Wen, Xuehan Zhang, Zhengyu Yu, Junming Zhou, Aihua Mao, Lixin Lv, Yiyi Hu, Yang Yu, Haodan Zhu, Xiaomin Wang. Complete genome sequence of a recombinant porcine epidemic diarrhea virus strain from eastern China. Genome Announc. 2013, 18;1(2): e00105-13.

39. Jianbo Cao, Bin Li (co-first author), Liurong Fang, Huanchun Chen, Shaobo Xiao. Pathogenesis of nonsuppurative encephalitis caused by highly pathogenic Porcine reproductive and respiratory syndrome virus. J Vet Diagn Invest. 2012, 24(4):767-71.

40. Bin Li*, Ma Junjie, Liu Yanling, Wen Libin, Yu Zhengyu, Ni Yanxiu, Zhang Xuehan, Zhou Junming, Guo Rongli, Wang Xiaomin, Feng Zhixin, Hu Yiyi, Lv Lixin, He Kongwang. Complete genome sequence of a highly prevalent porcine circovirus 2 isolated from piglet stool samples in China,J. Virol, 2012, 86(8), 4716.

41. Bin Li, Ma JJ, Xiao SB, Fang LR, Zeng SL, Wen LB, Zhang XH, Ni YX, Guo RL, Yu ZY, Zhou JM, Mao AH, Lv LX, Wang XM, He KW. Complete genome sequence of a novel species of porcine bocavirus, PBoV5. J. Virol. 2012, 86(2):1286–1287.

42. Bin Li, Ma JJ, Xiao SB, Wen LB, Ni YX, Zhang XH, Fang LR, He KW. Genome sequence of a highly prevalent porcine partetravirus in mainland China. J. Virol. 2012, 86:1899.

43. Bin Li, Ma JJ, Xiao SB, Zhang XH, Wen LB, Mao L, Ni YX, Guo RL, Zhou JM, Lv LX, He KW. Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. J. Virol. Methods. 2012, 179:390-395.

44. Bin Li, Liurong Fang, Xueliang Guo, Janfeng Gao, Tao Song, Jing Bi, Kongwang He, Huanchun, Chen, Shaobo Xiao. Epidemiology and Evolutionary Characteristics of the Porcine Reproductive and Respiratory Syndrome Virus in China between 2006 and 2010. J Clin Microbiol. 2011, 49 (9): 3175-3183.

45. Bin Li, Xiao SB, Ma JJ, Liu YL, Mao L, Wen LB, Mao AH, Zhang XH, Ni YX, Guo RL, Zhou JM, Yu ZY, Lv LX, Wang XM, Fang LR, Chen HC, He KW. Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV). Virol. J. 2011, 8:357.

46. Bin Li, Liurong Fang, Suyan Liu, Fuwei Zhao, Yunbo Jiang, Kongwang He, Huanchun Chen, Shaobo Xiao The genomic diversity of Chinese porcine reproductive and respiratory syndrome virus isolates from 1996 to 2009. Vet Microbiol. 2010, 146: 226-237.

47. Bin Li, Liurong Fang, Zuofei Xu, Suyan Liu, Jianfeng Gao, Yunbo Jiang, Huanchun Chen, Shaobo Xiao. Recombination in vaccine and circulating strains of porcine reproductive and respiratory syndrome virus. Emerg Infect Dis. 2009, 15 (12): 2032-2035.

48. Bin Li, Shaobo Xiao, Yanwei Wang, Shanshan Xu, Yunbo Jiang, Huanchun Chen, Liurong Fang. Immunogenicity of the highly pathogenic porcine reproductive and respiratory syndrome virus GP5 protein encoded by a synthetic ORF5 gene. Vaccine. 2009, 27: 1957-1963.

49. 武琦,周金柱,钟纯燕,李基棕,李科茫,索朗斯珠,贡嘎,李彬*。牛星状病毒ORF2基因遗传进化分析及原核表达。畜牧与兽医. 2022,54(10):81-88.

50. 王丹丹,周俊明,倪艳秀,祝昊丹,朱雪蛟,周金柱,李彬*。胞内劳森氏菌间接ELISA抗体检测方法的建立及应用.中国预防兽医学报.2022,44(04):396-401.

51. 宋诗莹,郭玮璐,夏学峰,张雪,毕振威,张雪寒,范宝超,董海龙,李彬。猪IFN-δ5的表达纯化及其抗PEDV感染的作用分析。中国畜牧兽医. 2022,49(08): 3171-3179.

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53. 时丹怡,范宝超,常新见,黄伟,李彬*。新型冠状病毒(SARS-CoV-2)与常见家养动物冠状病毒的同源性分析[J].江苏农业学报,2020,36(01):251-253.

54. 常新见,周金柱,鲁天弈,范宝超,郭容利,朱琳,李彬,牛家强,何孔旺*。 华东地区猪A群轮状病毒的VP7和VP4基因序列分析。中国兽医杂志,2019,55(10) :3-7+12.

55. 常新见,周金柱,范宝超,郭容利,朱琳,赵永祥,牛家强,何孔旺,李彬*。 猪轮状病毒NJ2012株VP8基因序列分析及原核表达。畜牧与兽医,2020,52(10) :68-72.

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57. 朱琳,孙冰,孙杰,郭容利,范宝超,李彬,索朗斯珠*,何孔旺*.猪流行性腹泻病毒JS2008株的传代培养及遗传变异分析[J].南方农业学报,2018,49(09):1833-1840.

58. 焦点,孙杰,俞正玉,郭容利,朱琳,范宝超,李郁*,何孔旺*,李彬.江苏及周边地区猪流行性腹泻病毒的检测及其ORF3基因序列分析[J].畜牧与兽医,2018,50(10):88-94.

59. 焦点,孙杰,茅爱华,俞正玉,郭容利,朱琳,范宝超,袁万哲,李郁,何孔旺,李彬*.2014-2016年我国江苏地区猪流行性腹泻病毒S基因的序列分析[J].中国兽医杂志,2018,54(06):8-11+15.

60. 俞正玉,逄凤娇,孙冰,徐向伟,茅爱华,郭容利,范宝超,杜露平,温立斌,倪艳秀,何孔旺,李彬*.猪流行性腹泻病毒变异株RT-PCR检测方法的建立[J].江苏农业科学,2018,46(01):116-118.

61. 孙冰,孙杰,徐向伟,俞正玉,郭容利,袁万哲,何孔旺,李彬*。猪流行性腹泻病毒间接 ELISA 检测方法的建立。中国兽医科学 2017,47(10) :1245-1249.

62. 孙杰,朱琳,焦点,俞正玉,范宝超,袁万哲,王建辉,郭容利,何孔旺,李彬*。 猪肠道病毒甲型 3C 基因的克隆和原核蛋白的表达。中国兽医科学 2017,47(10) :1287-1291.

63. 逄凤娇,俞正玉,何孔旺,徐向伟,张柏猛,郭容利,温立斌,袁万哲,姜平,李彬*. 猪丁型冠状病毒重组N蛋白间接ELISA抗体检测方法的建立。中国预防兽医学报. 2017,06(39):461-465.

64. 徐向伟,李彬,孙冰,郭容利,俞正玉,范宝超,姜平,倪艳秀,何孔旺。猪传染性胃肠炎病毒重组 N 蛋白间接 ELISA抗体检测方法的建立。畜牧与兽医,2016, 48(5):31-38.

65. 逄凤娇,俞正玉,何孔旺,徐向伟,郭容利,温立斌,姜平,李彬*. 猪Deltacoronavirus RT- PCR 检测方法的建立及其应用.中国预防兽医学报. 2015,37(9):683-686.

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72. 李彬,马俊杰,毛立,温立斌,张雪寒,倪艳秀,周俊明,何孔旺。猪Hokovirus PCR检测方法的建立及其应用。中国预防兽医学报,2012,34(2):108-111。

73. 李彬,马俊杰,赵静静,何孔旺,倪艳秀,温立斌,张雪寒,周俊明。断奶仔猪多系统衰竭综合征中多病原混合感染的流行病学调查。华北农学报,2012,27(5):203-206

74. 李彬,何孔旺,温立斌,茅爱华,王小敏,张雪寒,周俊明,肖少波,陈焕春. 我国长江流域PRRSV ORF3基因的遗传变异分析. 华北农学报. 2011, 26(2):1-6.

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